دانلود مقاله ISI انگلیسی شماره 137643
کد مقاله سال انتشار مقاله انگلیسی ترجمه فارسی تعداد کلمات
137643 2018 36 صفحه PDF سفارش دهید محاسبه نشده
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عنوان انگلیسی
Visualization of DNA in highly processed botanical materials
منبع

Publisher : Elsevier - Science Direct (الزویر - ساینس دایرکت)

Journal : Food Chemistry, Volume 245, 15 April 2018, Pages 1042-1051

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چکیده انگلیسی

DNA-based methods have been gaining recognition as a tool for botanical authentication in herbal medicine; however, their application in processed botanical materials is challenging due to the low quality and quantity of DNA left after extensive manufacturing processes. The low amount of DNA recovered from processed materials, especially extracts, is “invisible” by current technology, which has casted doubt on the presence of amplifiable botanical DNA. A method using adapter-ligation and PCR amplification was successfully applied to visualize the “invisible” DNA in botanical extracts. The size of the “invisible” DNA fragments in botanical extracts was around 20–220 bp compared to fragments of around 600 bp for the more easily visualized DNA in botanical powders. This technique is the first to allow characterization and visualization of small fragments of DNA in processed botanical materials and will provide key information to guide the development of appropriate DNA-based botanical authentication methods in the future.

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