|کد مقاله||سال انتشار||مقاله انگلیسی||ترجمه فارسی||تعداد کلمات|
|145372||2018||7 صفحه PDF||سفارش دهید||4425 کلمه|
Publisher : Elsevier - Science Direct (الزویر - ساینس دایرکت)
Journal : LWT, Volume 90, April 2018, Pages 448-454
Studies on the effects of electric fields on enzymatic activity have typically focused on post-treatment residual activity. However, information on their effects on in-situ activity is scant. The objective of this study was to investigate the effect of controlled-frequency Moderate Electric Fields (MEF) on Î±-amylase activity. Relative Î±-amylase activities were measured in-situ in the presence of an electric field (1â¯V/cm, 1â¯Hz-1MHz) at 60â¯Â°C, with a zero electric field at the same temperature serving as a control treatment. Results show a significant nonthermal effect of electric field frequency on Î±-amylase activity, with up to 41% enhancement of the activity in the 1â60â¯Hz frequency window. At higher frequencies, activity was either unaffected, or slightly inhibited by the presence of the electric field. Molecular dynamics simulation results show that in the 1â60â¯Hz frequency window, the translational electrophoretic enzyme displacement approaches the intermolecular spacing of water. Interestingly, enzyme rotational motion transitions from oscillatory behavior to full rotation below 60â¯Hz. Application of higher frequencies restricts the electrophoretic displacement of the enzyme due to rapid reversal of the direction of the electric field.