دانلود مقاله ISI انگلیسی شماره 149276
کد مقاله سال انتشار مقاله انگلیسی ترجمه فارسی تعداد کلمات
149276 2018 9 صفحه PDF سفارش دهید 6299 کلمه
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عنوان انگلیسی
Designing probe from E6 genome region of human Papillomavirus 16 for sensing applications
منبع

Publisher : Elsevier - Science Direct (الزویر - ساینس دایرکت)

Journal : International Journal of Biological Macromolecules, Volume 107, Part B, February 2018, Pages 1738-1746

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چکیده انگلیسی

Human Papillomavirus (HPV) is a standout amongst the most commonly reported over 100 types, among them genotypes 16, 18, 31 and 45 are the high-risk HPV. Herein, we designed the oligonucleotide probe for the detection of predominant HPV type 16 for the sensing applications. Conserved amino acid sequences within E6 region of the open reading frame in the HPV genome was used as the basis to design oligonucleotide probe to detect cervical cancer. Analyses of E6 amino acid sequences from the high-risk HPVs were done to check the percentage of similarity and consensus regions that cause different cancers, including cervical cancer. Basic local alignment search tools (BLAST) have given extra statistical parameters, for example, desire values (E-values) and score bits. The probe, ‘GGG GTC GGT GGA CCG GTC GAT GTA’ was designed with 66.7% GC content. This oligonucleotide probe is designed with the length of 24 mer, GC percent is between 40 and 70, and the melting point (Tm) is above 50 °C. The probe needed an acceptable length between 22 and 31 mer. The choice of region is identified here can be used as a probe, has implications for HPV detection techniques in biosensor especially for clinical determination of cervical cancer.

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