دانلود مقاله ISI انگلیسی شماره 30754
عنوان فارسی مقاله

ارتباط گیرنده پلی مورفیسم Tachykinin 2 TACR2 با بیماری آلزایمر

کد مقاله سال انتشار مقاله انگلیسی ترجمه فارسی تعداد کلمات
30754 2011 2 صفحه PDF سفارش دهید محاسبه نشده
خرید مقاله
پس از پرداخت، فوراً می توانید مقاله را دانلود فرمایید.
عنوان انگلیسی
No association of Tachykinin receptor 2 (TACR2) polymorphisms with Alzheimer's disease
منبع

Publisher : Elsevier - Science Direct (الزویر - ساینس دایرکت)

Journal : Neurobiology of Aging, Volume 32, Issue 3, March 2011, Pages 544–545

کلمات کلیدی
' بیماری آلزایمر - ژنتیک - 2 -
پیش نمایش مقاله
پیش نمایش مقاله ارتباط گیرنده پلی مورفیسم Tachykinin 2 TACR2 با بیماری آلزایمر

چکیده انگلیسی

The Tachykinin Receptor 2 (TACR2) located at chromosome 10q21.3 belongs to a class of receptors that bind members of the tachykinin neurotransmitter family. The TACR2 binds neurokinin A, also known as substance K, and is expressed in distinct parts of the human brain. Functionally, the TACR2 has been implicated in stress induced hippocampal acetylcholine release and the gene TACR2 is located within a previously identified linkage region for Alzheimer's disease (AD) on chromosome 10q21. Together, both facts make the TACR2 a reasonable positional and functional candidate gene for AD. Genotyping of 13 single nucleotide polymorphisms (SNPs) covering the entire gene and haplotypic analysis revealed no association with AD. Thus, we conclude that TACR2 can be excluded as a major susceptibility gene conferring risk to AD.

مقدمه انگلیسی

Alzheimer's disease is a complex neurodegenerative disease that is associated with a variety of genetic and environmental risk factors. However, to date, only one undisputed susceptibility gene has been identified, being the ε4 allele of APOE ( Saunders et al., 1993). Previously published linkage studies highlighted a linkage region to AD on chromosome 10q21 that includes the Tachykinin Receptor 2 gene (TACR2 MIM: 162321) ( Ertekin-Taner et al., 2000 and Myers et al., 2000). Additionally, TACR2 is highly expressed in the medial septum/diagonal band (MSDB), which is known for its cholinergic and GABA-ergic projections to the hippocampus. Atrophy of the septohippocampal cholinergic neurons of the MSDB has been reported in neurodegenerative disorders that are associated with cognitive decline, such as AD ( Morozova et al., 2008). Furthermore, it was shown that intraseptal infusions of TACR2 agonists and antagonists increase or block hippocampal acetylcholine release in rats, respectively ( Desvignes et al., 2003). As a consequence the TACR2 gene represents a reasonable functional and positional susceptibility gene altering the risk for AD. To comprehensively investigate the genetic role of TACR2, we performed a whole gene approach, genotyped 13 SNPs, including 7 tagging SNPs from HapMap, covering the complete genetic region of the TACR2 gene (see Supplementary material—Table 1) with an intermarker distance of 3.1 kb, determined the linkage disequilibrium (LD) structure, and explored single marker and haplotype associations using Haploview ( Barrett et al., 2005) or appropriate regression models. This was undertaken in a thoroughly diagnosed case-control cohort, consisting of individuals clinically diagnosed with sporadic AD (n=479; mean age onset, 69.1 ± 9.1 years) and cognitively healthy, age, gender and ethnicity matched population-based controls (n = 322; 66.7 ± 12.0 years). Only marker rs12771121 showed a weak association signal (p = 0.031; dominant model), but this signal did not survive correction for multiple testing. The LD-block structure (see Supplementary material—Figure 1), according to Gabriel et al. ( Gabriel et al., 2002), revealed three haplotype blocks at the TACR2 region, without significant association for any haplotype combination (see Supplementary material—Table 2). TACR2 represents a reasonable positional and functional candidate gene for AD. The LD-structure of the 13 genotyped SNPs shows high r2-values except for rs12250793, which is not informative due to a minor allele frequency of 0.002. The marker rs3793853, when compared to the HapMap LD-structure of this region, is located next to a recombination hot spot indicated by low r2 and D’ values. Based on the LD-structure in our collective, similar to that observed in the HapMap collective, the fact that we did not find evidence of a significant association of common TACR2 variants with AD suggests that it is unlikely that we failed to identify a possible association due to an untyped variant. Likewise with statistical power, as a power analysis revealed that, at a significance level of α = 0.05, we had a power of 91% to detect a risk allele of 15% frequency mediating a relative risk of 1.6. However, as the statistical power drops considerably with decreasing minor allele frequencies and in particular for rare variants a possible association of these variants cannot be ruled out. In summary, this study does not provide strong evidence that common genetic variations of TACR2 are associated with an increased risk to develop AD. Due to the limited sample size, our genetic findings do not rule out a possible genetic association of rare TACR2 variants and a functional involvement of TACR2 in the pathogenesis of neurodegeneration in AD.

خرید مقاله
پس از پرداخت، فوراً می توانید مقاله را دانلود فرمایید.