انتقال ژن لپتین در هیپوتالاموس باعث افزایش طول عمر در موش جهش یافته های تک ژنی بزرگسالان در غیاب لپتین در گردش می شود
|کد مقاله||سال انتشار||مقاله انگلیسی||ترجمه فارسی||تعداد کلمات|
|38085||2007||11 صفحه PDF||سفارش دهید||محاسبه نشده|
Publisher : Elsevier - Science Direct (الزویر - ساینس دایرکت)
Journal : Neurobiology of Aging, Volume 28, Issue 10, October 2007, Pages 1594–1604
Leptin, a product of the ob gene, is a pleiotropic signal implicated in regulation of multiple physiological functions in the periphery and centrally, including hypothalamic integration of energy homeostasis. Recessive mutations of ob gene result in early onset of hyperphagia, morbid obesity, metabolic disorders, early mortality and shortened life-span. Intracerebroventricular injection of recombinant adeno-associated virus vector (rAAV) encoding the leptin gene in adult obese ob/ob mice enhanced leptin transgene expression only in the hypothalamus, normalized food intake, body weight and more than doubled the life-span as compared to control cohorts and extended it to near that of normal wild type mice. These life-extending benefits were associated with drastic reductions in visceral fat, and blood glucose and insulin levels, but elevated ghrelin levels, the anti-aging biomarkers. Thus, bioavailability of leptin transduced by ectopic gene in the hypothalamus alone is both necessary and sufficient to normalize life-span. Evidently, site-specific ectopic gene expression with rAAV is durable and safe for alleviating neural disorders that stem from missing or functional disruption of a single gene.
Environmental and genetic causes either alone or in a complex interplay are thought to contribute to the current worldwide escalation in the incidence of obesity and obesity-dependent metabolic and neurological afflictions , , , , , ,  and . Genetically-based disruptions that tilt energy balance in favor of increased energy consumption, decreased energy expenditure and accelerated rates of fat deposition are diverse and involve complex interactions of neural, hormonal and metabolic factors , , , , ,  and . Recent delineation of these varied derangements in the internal environment in monogenic and polygenic obesities has uncovered avenues for therapeutic interventions at genetic and molecular levels , ,  and . Rodent models of monogenic obesity are suitable paradigms to test novel molecular and genetic interventional approaches aimed at retarding fat accretion on a long-term basis , ,  and . Recessive mutation in ob gene results in morbid obesity resulting from an early onset of hyperphagia and decreased energy expenditure , , , ,  and . Leptin, the product of the ob gene, is produced by adipocytes and non-adipocyte tissues, including the hypothalamus , , , , ,  and . Leptin is a pleiotropic signal that regulates multiple physiological functions in the periphery and neural functions in the brain by engaging target specific leptin receptors , , ,  and . In the periphery, leptin has been implicated in the regulation of blood pressure, renal function, angiogenesis, wound healing, immune function and bone formation , , , , , , , ,  and . Among central effects, a primary action of leptin is to integrate energy homeostasis by controlling distinct energy intake and expenditure regulating neural pathways in the hypothalamus , , ,  and . Absence of leptin engenders intense hyperphagia, excess fat accretion, life-long morbid obesity accompanied by diminished non-shivering thermogenic energy expenditure in leptin-mutant ob/ob mice and congenital leptin-deficient human subjects , , , , , , , ,  and . Morbidly obese human patients and obese mice exhibit a variety of life-threatening complications, including metabolic syndrome, early mortality and shortened life-span , , , , , , , ,  and . Experimental evidence showing that leptin replacement either systemically or centrally restores energy homeostasis in ob/ob mice , ,  and , and conditional deletion of leptin receptors in the hypothalamus reproduces the ob/ob-type phenotype ,  and , suggests that leptin action in the hypothalamus alone can reinstate weight homeostasis in ob/ob mice. However, whether imbalance due to leptin deficiency in the hypothalamus and/or global pathophysiologic complications resulting from leptin deficiency in the periphery accelerate aging and shorten life-span, is not known. Research in gene transfer strategies for developing interventional therapies for neural disorders has proceeded at rapid pace , ,  and . Among various gene therapy approaches currently available, insertion of a missing gene in an appropriate site with the aid of a suitable viral vector has the potential to remedy the pathophysiologic consequences of a mutant gene on a long-term basis , ,  and . Leptin gene transfer into the hypothalamus with a non-pathogenic and non-immunogenic recombinant adeno-associated virus encoding the leptin gene (rAAV-lep), was found to enhance leptin transgene expression in the hypothalamus contemporaneous with weight normalization, decreased adiposity and symptoms of type 2 diabetes in leptin-mutant ob/ob mice even in the absence of leptin into the periphery , , , , , , , , , , , ,  and . A similar central leptin gene therapy retarded the gradual time-related increase in fat accretion and secretion of adipocyte adipokines in wild type (wt) mice in short term experiments ,  and . Since rAAV vectors infect mainly neurons in the nervous system and support expression of targeted gene for the lifetime of cells in vivo ,  and , we examined the efficacy of bioavailability of leptin by leptin gene transfer in the hypothalamus on mortality and longevity in ob/ob mice.