دانلود مقاله ISI انگلیسی شماره 47176
عنوان فارسی مقاله

پایش محیطی جریان شهری با استفاده از سیستم کشت سلولی اولیه آبشش ماهی (FIGCS)

کد مقاله سال انتشار مقاله انگلیسی ترجمه فارسی تعداد کلمات
47176 2015 7 صفحه PDF سفارش دهید محاسبه نشده
خرید مقاله
پس از پرداخت، فوراً می توانید مقاله را دانلود فرمایید.
عنوان انگلیسی
Environmental monitoring of urban streams using a primary fish gill cell culture system (FIGCS)
منبع

Publisher : Elsevier - Science Direct (الزویر - ساینس دایرکت)

Journal : Ecotoxicology and Environmental Safety, Volume 120, October 2015, Pages 279–285

کلمات کلیدی
متالوتیونین - ماهی - پایش محیطی - کشت سلول
پیش نمایش مقاله
پیش نمایش مقاله پایش محیطی جریان شهری با استفاده از سیستم کشت سلولی اولیه آبشش ماهی  (FIGCS)

چکیده انگلیسی

The primary fish gill cell culture system (FIGCS) is an in vitro technique which has the potential to replace animals in whole effluent toxicity tests. In the current study FIGCS were transported into the field and exposed to filtered (0.2 μm) river water for 24 h from 4 sites, on 2 different sampling dates. Sites 1 and 2 are situated in an urban catchment (River Wandle, London, UK) with site 1 downstream of a sewage treatment work; site 3 is located in a suburban park (River Cray, Kent, UK), and site 4 is more rural (River Darent, Kent, UK). The change in transepithelial electrical resistance (TER), the expression of the metal responsive genes metallothionein A (mta) and B (mtb), cytochrome P450 1A1 (cyp1a1) and 3A27 (cyp3a27), involved in phase 1 metabolism, were assessed following exposure to sample water for 24 h. TER was comparable between FIGCS exposed to 0.2 μm filtered river water and those exposed to synthetic moderately soft water for 24 h. During the first sampling time, there was an increase in mta, cyp1a1 and cyp3a27 gene expression in epithelium exposed to water from sites 1 and 2, and during the second sampling period an increase in cyp3a27 gene expression at sites 1 and 4. Urban river water is a complex mixture of contaminants (e.g., metals, pesticides, pharmaceuticals and polyaromatic hydrocarbons) and the increase in the expression of genes encoding mta, cyp1a1 and cyp3a27 in FIGCS is indicative of the presence of biologically active pollutants.

خرید مقاله
پس از پرداخت، فوراً می توانید مقاله را دانلود فرمایید.