دانلود مقاله ISI انگلیسی شماره 116796
ترجمه فارسی عنوان مقاله

ارزیابی واکسن های واکسیناسیون از واکسن داخل بینی و ایمنی کمکی برای آزمایشات پیش از موعد

عنوان انگلیسی
A novel vaccinological evaluation of intranasal vaccine and adjuvant safety for preclinical tests
کد مقاله سال انتشار تعداد صفحات مقاله انگلیسی
116796 2017 10 صفحه PDF
منبع

Publisher : Elsevier - Science Direct (الزویر - ساینس دایرکت)

Journal : Vaccine, Volume 35, Issue 5, 1 February 2017, Pages 821-830

پیش نمایش مقاله
پیش نمایش مقاله  ارزیابی واکسن های واکسیناسیون از واکسن داخل بینی و ایمنی کمکی برای آزمایشات پیش از موعد

چکیده انگلیسی

Vaccines are administered to healthy humans, including infants, so the safety and efficacy must be very high. Therefore, evaluating vaccine safety in preclinical and clinical studies, according to World Health Organization guidelines, is crucial for vaccine development and clinical use. A change in the route of administration is considered to alter a vaccine’s immunogenicity. Several adjuvants have also been developed and approved for use in vaccines. However, the addition of adjuvants to vaccines may cause unwanted immune responses, including facial nerve paralysis and narcolepsy. Therefore, a more accurate and comprehensive strategy must be used to develope next-generation vaccines for ensuring vaccine safety. Previously, we have developed a system with which to evaluate vaccine safety in rats using a systematic vaccinological approach and 20 marker genes. In this study, we developed a safety evaluation system for nasally administered influenza vaccines and adjuvanted influenza vaccines using these marker genes. Expression of these genes increased dose-dependent manner when mice were intranasally administered the toxicity reference vaccine. When the adjuvant CpG K3 or a CpG-K3-combined influenza vaccine was administered intranasally, marker gene expression increased in a CpG-K3-dose-dependent way. A histopathological analysis indicated that marker gene expression correlated with vaccine- or adjuvant-induced phenotypic changes in the lung and nasal mucosa. We believe that the marker genes expression analyses will be useful in preclinical testing, adjuvant development, and selecting the appropriate dose of adjuvant in nasal administration vaccines.