بیان سیگنال هیدروژن سولفید درونی در هنگام استئوژنز منحرف کردن در یک مدل خرگوش

The hydrogen sulfide (H2S) signal system plays an important role in bone metabolism. However, the role of endogenous H2S during distraction osteogenesis (DO) remains unclear. Sixty-two male New Zealand White rabbits were subjected to right mandibular DO. Before distraction, the animals were divided randomly into two groups: group A, 0.5 mm twice/day for 10 days; group B, 1.25 mm twice/day for 4 days. Plasma and distraction gap tissue were harvested to determine the H2S signal. The osteogenesis effect was also evaluated. The newly regenerated bone in group A presented a higher level of mineralization and biomechanical strength than that in group B. The bone mineralization density in group A was 1.95-fold that in group B (P = 0.028), while the biomechanical strength in group A was 1.26-fold that in group B (P = 0.042) at the end of the experiment. The H2S signal was detected during the whole process of DO. The relative plasma H2S concentrations in group A were noticeably higher than those in group B at the middle of distraction (P < 0.001), at the end of the distraction (P = 0.034), and 2 weeks after the end of distraction (P = 0.002). The results suggest that the endogenous H2S signal system plays a major role during DO.