مرجع فضایی- (کارکردی یا رویه ای-) عملکرد حافظه موش های سالم در ماز آب پیش بینی می کند که تسهیل آزادی استیل کولین ناشی از سولپریاید با استفاده از برش های ناهنجار

Cluster analysis of water-maze reference-memory performance distinguished subpopulations of young adult (3–5 months), aged (25–27 months) unimpaired (AU) and aged impaired (AI) rats. Working-memory performances of AU and AI rats were close to normal (though young and aged rats differed in exploration strategies). All aged rats showed impaired procedural-memory. Electrically evoked release of tritium was assessed in striatal slices (preloaded with [3H]choline) in the presence of oxotremorine, physostigmine, atropine + physostigmine, quinpirole, nomifensine or sulpiride. Aged rats exhibited reduced accumulation of [3H]choline (−30%) and weaker transmitter release. Drug effects (highest concentration) were reductions of release by 44% (oxotremorine), 72% (physostigmine), 84% (quinpirole) and 65% (nomifensine) regardless of age. Sulpiride and atropine + physostigmine facilitated the release more efficiently in young rats versus aged rats. The sulpiride-induced facilitation was weaker in AI rats versus AU rats; it significantly correlated with reference-memory performance. The results confirm age-related alterations of cholinergic and dopaminergic striatal functions, and point to the possibility that alterations in the D2-mediated dopaminergic regulation of these functions contribute to age-related reference-memory deficits.