دانلود مقاله ISI انگلیسی شماره 98790
ترجمه فارسی عنوان مقاله

بیان انقراض از آنتی بادی های محلول در دمای پایین و بیش از حد با منبع نیتروژن

عنوان انگلیسی
Enhanced expression of soluble antibody fragments by low-temperature and overdosing with a nitrogen source
کد مقاله سال انتشار تعداد صفحات مقاله انگلیسی
98790 2018 7 صفحه PDF
منبع

Publisher : Elsevier - Science Direct (الزویر - ساینس دایرکت)

Journal : Enzyme and Microbial Technology, Volume 115, August 2018, Pages 9-15

ترجمه کلمات کلیدی
قطعه آنتی بادی، محلول فاب، دمای پایین، تخمیر بذر فدرال، اثر نیتروژن،
کلمات کلیدی انگلیسی
Antibody fragment; Soluble Fab; Low-temperature; Fed-batch fermentation; Nitrogen effect;
پیش نمایش مقاله
پیش نمایش مقاله  بیان انقراض از آنتی بادی های محلول در دمای پایین و بیش از حد با منبع نیتروژن

چکیده انگلیسی

Escherichia coli has been a primary host for the prokaryotic production of antibody fragments (Fabs) and has contributed to several successes in the pharmaceutical industry. Nevertheless, the requirement of disulfide bonds often results in low-yield fermentation and a lack of cost-effectiveness. Despite the improved production of functional Fabs by fermentation below 30 °C, the limited cellular growth needs further work. To address these issues, we investigated the effect of nitrogen supply on the cellular growth and the Fab productivity. We used the anti-human VEGF-A Fab as a model that exhibited poor expression at 37 °C regardless of the amount of nitrogen supplied during fermentation. In stark contrast, the expression yield of soluble Fab with a gross nitrogen supply of 6.91 g/L of broth throughout the fermentation at 25 °C was 332 mg/L. Furthermore, and increased nitrogen supply of 10.9 g/L significantly improved the yield of active form by 59.7% and the cellular growth rate by 39.3%. These results indicate that overdosing of a nitrogen source at low temperature is critical to Fab productivity in E. coli.