فوکوس اتوماتیک میکروسکوپ پرتو ایری با فاصله کانونی بلند

Stimulated emission depletion (STED) is a very important technique in super-resolution microscopy. Until now, while autofocusing Airy beam (AAB) has been an attractive theme for both theoretical and applied researches, there are almost no report on AABs being used in STED microscopy. In this paper, we propose a novel STED microscopy based on AABs. A radially symmetric 3/2 phase plate is involved to simultaneously generate autofocusing excitation- and depletion-Airy beams. Remarkably, the AAB can auto-focus to a wavelength-scale spot with a long focal depth (several millimeters): on the contrary, the working distance of a conventional high numerical aperture (NA) objective is usually very short (about 200   μm). Our calculations indicate that the AAB based STED microscopy can achieve a super-resolution spot with FWHM of 58  nm while the focal length is 4.638  mm. Moreover, with properties of non-diffracting and self-healing, the Airy beam could enable a reduction of the scattering distortion induced by the specimens and has a great potential in imaging thick specimens.